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hek gp2 293  (TaKaRa)


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    Structured Review

    TaKaRa hek gp2 293
    Hek Gp2 293, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hek+gp2+293+cells/HEK+293+Tet-Off+Advanced+Cell+Line/pmc09426521-184-5-7
    Average 94 stars, based on 175 article reviews
    hek gp2 293 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Retroviral:

    Article Title: Constrained G4 structures unveil topology specificity of known and new G4 binding proteins
    Article Snippet: The pLPC-puro-N-Flag-NELFE plasmid was obtained by amplification of the human NELFE cDNA (obtained from gene synthesis, GeneArt, LifeTechnlogies) using oligonucleotides: Fwd 5’-GACGATGACGATAAAGGATCCTTGGTGATACCCCCCGGACT-3’ and Rev-5’CCCTCTAGATGCATGCTCGAGCTAGAAGCCATCCACAAGGTTTTCC-3’ and inserted in the pLPC-puro-N-Flag plasmid between XhoI and BamH1 restriction sites. .. Retroviral production was performed by transient transfection of HEK-GP2 293 cells (Clontech) with 0.8 μg pCMVsVg and 1.2 μg of pLPC-puro-N-Flag-NELFE plasmids using JetPrime reagent (polyplus). ..

    Article Title: Constrained G4 structures unveil topology specificity of known and new G4 binding proteins
    Article Snippet: The pLPC-puro-N-Flag-NELFE plasmid was obtained by amplification of the human NELFE cDNA (obtained from gene synthesis, GeneArt, LifeTechnlogies) using oligonucleotides: Fwd 5′-GACGATGACGATAAAGGATCCTTGGTGATACCCCCCGGACT-3′ and Rev- 5′CCCTCTAGATGCATGCTCGAGCTAGAAGCCATCCACAAGGTTTTCC-3′ and inserted in the pLPC-puro-N-Flag plasmid between XhoI and BamH1 restriction sites. .. Retroviral production was performed by transient transfection of HEK-GP2 293 cells (Clontech) with 0.8 μg pCMVsVg and 1.2 μg of pLPC-puro-N-Flag-NELFE plasmids using JetPrime reagent (polyplus). ..

    Transfection:

    Article Title: Constrained G4 structures unveil topology specificity of known and new G4 binding proteins
    Article Snippet: The pLPC-puro-N-Flag-NELFE plasmid was obtained by amplification of the human NELFE cDNA (obtained from gene synthesis, GeneArt, LifeTechnlogies) using oligonucleotides: Fwd 5’-GACGATGACGATAAAGGATCCTTGGTGATACCCCCCGGACT-3’ and Rev-5’CCCTCTAGATGCATGCTCGAGCTAGAAGCCATCCACAAGGTTTTCC-3’ and inserted in the pLPC-puro-N-Flag plasmid between XhoI and BamH1 restriction sites. .. Retroviral production was performed by transient transfection of HEK-GP2 293 cells (Clontech) with 0.8 μg pCMVsVg and 1.2 μg of pLPC-puro-N-Flag-NELFE plasmids using JetPrime reagent (polyplus). ..

    Article Title: Constrained G4 structures unveil topology specificity of known and new G4 binding proteins
    Article Snippet: The pLPC-puro-N-Flag-NELFE plasmid was obtained by amplification of the human NELFE cDNA (obtained from gene synthesis, GeneArt, LifeTechnlogies) using oligonucleotides: Fwd 5′-GACGATGACGATAAAGGATCCTTGGTGATACCCCCCGGACT-3′ and Rev- 5′CCCTCTAGATGCATGCTCGAGCTAGAAGCCATCCACAAGGTTTTCC-3′ and inserted in the pLPC-puro-N-Flag plasmid between XhoI and BamH1 restriction sites. .. Retroviral production was performed by transient transfection of HEK-GP2 293 cells (Clontech) with 0.8 μg pCMVsVg and 1.2 μg of pLPC-puro-N-Flag-NELFE plasmids using JetPrime reagent (polyplus). ..



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